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Research Associate- Melenhorst laboratory

Philadelphia, PA, US, 19104

Job Type: 

Req ID: 25593

Shift: Days

Employment Status: AF - Active - Regular - Full Time 

Job Summary

Research Associate under the direction of Dr. Jos Melenhorst and close collaboration with Dr. Steve Grupp and Dr. David Barrett, assume the responsibility of the genetic, proteomic, and functional interrogation T cells from patients treated with CD19-directed chimeric antigen receptor (CAR)-engineered T cells for their B cell malignancies.

 

The Melenhorst laboratory has identified key biomarkers and mechanisms of response to CD19 CAR T cells in chronic lymphocytic leukemia (Fraietta et al., 2018, Nat Med 24(5):563-571; Fraietta et al., 2018, Nature 558(7709):307-312; Ruella et al., 2018, 24(10):1499-1503). Similar studies are warranted in other settings, utilizing different leukemia-targeting CAR and manufacturing designs. The scientist is expected to carry out such translational immunology studies, utilizing patient biospecimens collected at various post-infusion time points, from the peripheral blood, bone marrow, and lymph nodes, to map the fate of the CD19-redirected T cells utilizing high dimensionality tools such as bulk and single cell RNA and ATAC sequencing, mass cytometry, CITE-seq, plus flow cytometric sorting for additional genetic and functional studies of the purified T cells. In addition, the response of the tumor and tumor microenvironment to this form of immunogene therapy will be assessed using similar single-cell technologies, complemented with in situ analyses via immunohistochemistry with the ultimate aim to develop a deep understanding of the biology and to implement the findings into a new process to augment the clinical impact of CAR T cell therapies.

 

Key technologies that will be used for these studies include  bulk and single-cell RNA sequencing and ATAC sequencing analysis; flow and mass cytometry; metabolism studies using the Seahorse and other, high-content metabolic technologies; sorting of cell populations for detailed functional and genetic characterization; cell culture, employing various genome engineering technologies, including CRISPR/Cas9-mediated knock-out and homology-directed repair knock-in approaches; and xenogeneic mouse models using an established ALL model and patient-derived xenogeneic (PDX) models which will be developed for this project.

 

The Research Associate  is expected have a very strong background in human T cell immunology, specifically cell culture, flow and/or cytometry, gene engineering with retro-/lentiviral vectors, CRISPR/Cas9, and genetic analysis tools. Furthermore, the sicentist is expected to work very closely with a team of other scientists on related projects and, in collaboration with a bioinformatician develop the deep understanding needed to guide therapeutic augmentation of CAR T cells. The scientist is furthermore expected to write summary reports, papers, and give presentations at local and domestic scientific meetings.

 

Job Responsibilities

 
  • Assists faculty in the conduct of research of significant value in the basic and/or translational science area
  • Plans, conducts, and manages research projects within the federal and institutional regulations and policies
  • Collaborates with researchers, external agencies and institutions to develop cooperative research initiatives
  • Trains technical staff, students, fellows & physicians in lab procedures
  • Reviews manuscripts for peer-reviewed journals
  • Assist in composing/developing applications for grants/contracts for funding of research projects
  • Promotes free discussion, research progress, and overall morale
  • Responsible for appropriate use of research funds
  • May supervises staff and students

Required Licenses, Certifications, Registrations

None

Required Education and Experience

  • Master’s degree, 5-7 years of scientifically relevant experience required
  • Bachelor’s degree 10+ years of scientifically relevant experience required

 

Preferred Education, Experience & Cert/Lic

Terminal degree (MD, PhD, DVM) preferred, 2 or more years Post-Doctoral fellowship experience; 1-3 years scientifically relevant experience

Additional Technical Requirements

All CHOP employees who work in a patient building or who provide patient care are required to receive an annual influenza vaccine unless they are granted a medical or religious exemption.

Children's Hospital of Philadelphia is committed to providing a safe and healthy environment for its patients, family members, visitors and employees. In an effort to achieve this goal, employment at Children's Hospital of Philadelphia, other than for positions with regularly scheduled hours in New Jersey, is contingent upon an attestation that the job applicant does not use tobacco products or nicotine in any form and a negative nicotine screen (the latter occurs after a job offer).

Children's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.

VEVRAA Federal Contractor/Seeking priority referrals for protected veterans.  Please contact our hiring official with any referrals or questions.

CHOP Careers Contact 

Talent Acquisition

2716 South Street, 6th Floor

Philadelphia, PA 19146 

Phone: 866-820-9288 

Email:TalentAcquisition@email.chop.edu

 

 

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