Res Post Doc - Melenhorst laboratory

Philadelphia, PA, US, 19104

Job Type: 

Req ID: 26632

Shift: Days

Employment Status: AF - Active - Regular - Full Time 

Job Summary

Post-doctoral researcher under the direction of Dr. Jos Melenhorst and close collaboration with Dr. Steve Grupp and Dr. David Barrett, assume the responsibility of the genetic, proteomic, and functional interrogation T cells from patients treated with CD19-directed chimeric antigen receptor (CAR)-engineered T cells for their B cell malignancies.

The first wave of CAR T cell therapies have yielded dramatic responses in blood cancers, with wide-ranging efficacies and toxicities. We have identified key biomarkers predictive of severe adverse events such as cytokine release syndrome, plus the natural and synthetic basis of effective and ineffective CAR T cell therapies in leukemia. Similar studies are warranted in other settings, utilizing different leukemia-targeting CAR and manufacturing designs. The post-doc is expected to carry out such translational immunology studies, utilizing patient biospecimens collected at various post-infusion time points, from the peripheral blood, bone marrow, and lymph nodes, to map the fate of the CD19-redirected T cells utilizing high dimensionality tools such as bulk and single cell RNA and ATAC sequencing, mass cytometry, CITE-seq, plus flow cytometric sorting for additional genetic and functional studies of the purified T cells. In addition, the response of the tumor and tumor microenvironment to this form of immunogene therapy will be assessed using similar single-cell technologies, complemented with in situ analyses via immunohistochemistry with the ultimate aim to develop a deep understanding of the biology and to implement the findings into a new process to augment the clinical impact of CAR T cell therapies.

The post-doc will work closely with a research associate. She/he is furthermore part of a multidisciplinary team of translational scientists, geneticists, and bioinformaticians to develop the deep understanding needed to guide therapeutic augmentation of CAR T cells. The scientist is furthermore expected to write summary reports, papers, and give presentations at local and domestic scientific meetings.



Job Responsibilities


  • Conduct research of significant value in the basic and/or translational science area
  • Plans, conducts, and manages research projects within the federal and institutional regulations and policies
  • Collaborates with researchers, external agencies and institutions to develop cooperative research initiatives
  • Trains technical staff, students, fellows & physicians in lab procedures
  • Reviews manuscripts for peer-reviewed journals
  • Assist in composing/developing applications for grants/contracts for funding of research projects
  • Promotes free discussion, research progress, and overall morale
  • Responsible for appropriate use of research funds
  • May supervise staff and students 
Required Licenses, Certifications, Registrations
Required Education and Experience
  • A terminal degree PhD, MD, DVM, etc.
  • According to the CMS (Centers for Medicaid and Medicare Services) a copy of diploma or transcript is required as proof of academic achievement for all non-laboratory personnel performing non-waived POC testing
Preferred Education, Experience & Cert/Lic
Additional Technical Requirements

All CHOP employees who work in a patient building or who provide patient care are required to receive an annual influenza vaccine unless they are granted a medical or religious exemption.

Children's Hospital of Philadelphia is committed to providing a safe and healthy environment for its patients, family members, visitors and employees. In an effort to achieve this goal, employment at Children's Hospital of Philadelphia, other than for positions with regularly scheduled hours in New Jersey, is contingent upon an attestation that the job applicant does not use tobacco products or nicotine in any form and a negative nicotine screen (the latter occurs after a job offer).

Children's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.

VEVRAA Federal Contractor/Seeking priority referrals for protected veterans.  Please contact our hiring official with any referrals or questions.

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